KMID : 1161520200240040233
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Animal Cells and Systems 2020 Volume.24 No. 4 p.233 ~ p.241
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The immunostimulatory effect of indole-6-carboxaldehyde isolated from Sargassum thunbergii (Mertens) Kuntze in RAW 264.7 macrophages
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Park Cheol
Hwangbo Hyun Lee Hye-Sook Kim Gi-Young Cha Hee-Jae Choi Sung-Hyun Kim Suhk-Mann Kim Heui-Soo Yun Seok-Joong Kim Wun-Jae Jeon You-Jin Choi Yung-Hyun
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Abstract
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Indole-6-carboxaldehyde (I6CA), an indole derivative isolated from the marine brown algae Sargassum thunbergii, is known to have several beneficial effects, but no studies on immune regulation have been conducted. In this study, the immunomodulatory properties of I6CA on murine RAW 264.7 monocyte/macrophage cells were evaluated. As the concentration of I6CA increased, the morphology of RAW 264.7 cells changed to a typical active macrophage shape, and the phagocytic activity increased significantly. I6CA effectively enhanced the production and secretion of immunomodulatory mediators and cytokines due to increased expression of their respective genes. Additionally, I6CA markedly stimulated the expression of Toll-like receptor 4 (TLR4) and its adapter molecule, myeloid differentiation factor 88 (Myd88), and increased TLR4 complexed with Myd88. Furthermore, I6CA promoted the nuclear translocation of nuclear factor-kappa B (NF-¥êB) by increasing the degradation of the inhibitor of NF-¥êB-¥á. Meanwhile, similar trends were also found in lipopolysaccharide-treated cells as a positive control. Furthermore, molecular docking simulation showed that I6CA interacted with TLR4-myeloid differentiation 2 complex. Taken together, the results support the concept that I6CA may increase the activity of the TLR4/NF-¥êB signaling pathway in order to enhance the immunomodulatory activity of RAW 264.7 cells.
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KEYWORD
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Indole-6-carboxaldehyde, immunomodulatory property, TLR4, NF-¥êB
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